Coding

Part:BBa_K3602014:Design

Designed by: Flora Fuglsang   Group: iGEM20_SDU-Denmark   (2020-10-23)


Cas12a w. tags, optimized for E. coli K12


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2231
    Illegal BamHI site found at 1476
    Illegal XhoI site found at 1111
    Illegal XhoI site found at 1513
    Illegal XhoI site found at 2974
    Illegal XhoI site found at 3292
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 805
    Illegal AgeI site found at 2296
    Illegal AgeI site found at 3694
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 868


Design Notes

This sequence is designed with the 6x His Tag/Twin Strep/SUMO tags for purification and codon-optimized for the bacteria that is used for protein expression.


Source

This sequence is from the PhyLife group of the University of Southern Denmark, where it was originally used for human cells.

References